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dc.contributor.authorIzdebski, Radosław
dc.contributor.authorBaraniak, Anna
dc.contributor.authorHerda, Małgorzata
dc.contributor.authorFiett, Janusz
dc.contributor.authorBonten, Marc J.M.
dc.contributor.authorCarmeli, Yehuda
dc.contributor.authorGoossens, Herman J.
dc.contributor.authorHryniewicz, Waleria
dc.contributor.authorBrun- Buisson, Christian J.L.
dc.contributor.authorGniadkowski, Marek
dc.contributor.authorGrabowska, Anna
dc.contributor.authorNikonorow, Ewa
dc.contributor.authorDerde, Lennie P.G.
dc.contributor.authorDautzenberg, Mirjam J.D.
dc.contributor.authorAdler, Amos
dc.contributor.authorKazma, Meital
dc.contributor.authorNavon- Venezia, Shiri
dc.contributor.authorMalhotra-Kumar, Surbhi
dc.contributor.authorLammens, Christine
dc.contributor.authorDumpis, U.
dc.contributor.authorGiamarellou, Eleni
dc.contributor.authorMuzlovič, Igor
dc.contributor.authorNardi, Giuseppe
dc.contributor.authorPetrikkos, Georgios L.
dc.contributor.authorStammet, Pascal
dc.contributor.authorSalomon, Jérôme
dc.contributor.authorLawrence, Christine
dc.contributor.authorLegrand, Patrick
dc.contributor.authorRossini, A.
dc.contributor.authorSalvia, Antonino
dc.contributor.authorSamso, J. Vidal
dc.contributor.authorPaul, Mical
dc.contributor.authorLerman, Yaffa
dc.creatorIzdebski, Radosław
dc.date.accessioned2019-03-13T09:30:12Z
dc.date.available2019-03-13T09:30:12Z
dc.date.issued2015-01-01
dc.identifierSCOPUS_ID:84925118122
dc.identifier.issn03057453
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84925118122&origin=inward
dc.identifier.urihttps://repo.euc.ac.cy/handle/123456789/1969
dc.description.abstractObjectives: To perform the first multinational Enterobacter cloacae clonality study, using the MLST scheme newly developed in Japan. Methods: The analysis included 195 rectal carriage E. cloacae isolates resistant to expanded-spectrum cephalosporins (ESCs), collected from patients in 12 hospital units across Europe and Israel. All of the isolates were typed by PFGE and 173 isolates were subjected to MLST. ESC resistance was analysed phenotypically; genes encoding ESBLs and carbapenemases were identified by PCR and sequencing. Results: MLST distinguished 88 STs, which correlated with the PFGE data. PFGE was more discriminatory, producing 129 pulsotypes (169 patterns). Numerous STs were observed in several countries each. The most widespread were ST66, ST78, ST108 and ST114, each having at least 10 isolates from three to five countries, diversified into multiple pulsotypes, with clusters of related isolates in one or more centres. Analysis of the STs against the MLST database revealed several epidemic clonal complexes, such as those with central genotypes ST74 (including ST78) or ST114 (including ST66). ESC resistance was equally related to overexpression of the AmpC cephalosporinase and to ESBL production. Among ESBL producers some spreading subclones were identified, including specific ST66, ST78 and ST114 pulsotypes, associated with CTX-M-15 production. Several isolates produced carbapenemase VIM-1 or KPC-2. Conclusions: Together with the information available in the MLST database, our results suggest that, like Escherichia coli and Klebsiella pneumoniae, E. cloacae harbours clonal lineages of increased epidemic potential that may be associated with resistance spread.
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
dc.titleMLST reveals potentially high-risk international clones of Enterobacter cloacae
elsevier.identifier.doi10.1093/jac/dku359
elsevier.identifier.eid2-s2.0-84925118122
elsevier.identifier.scopusidSCOPUS_ID:84925118122
elsevier.volume70
elsevier.issue.identifier1
elsevier.coverdate2015-01-01
elsevier.coverdisplaydate1 January 2015
elsevier.openaccess1
elsevier.openaccessflagtrue
elsevier.aggregationtypeJournal


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