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dc.contributor.authorGkretsi, Vasiliki
dc.contributor.authorBowen, William C.
dc.contributor.authorYang, Yu Q.
dc.contributor.authorWu, Chuanyue
dc.contributor.authorMichalopoulos, George Konstantine
dc.creatorGkretsi, Vasiliki
dc.date.accessioned2018-11-27T12:49:15Z
dc.date.available2018-11-27T12:49:15Z
dc.date.issued2007-02-16
dc.identifierSCOPUS_ID:33845989065
dc.identifier.issn0006291X
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33845989065&origin=inward
dc.identifier.urihttps://repo.euc.ac.cy/handle/123456789/1042
dc.description.abstractHepatocytes have restricted proliferative capacity in culture and when cultured without matrix, lose the hepatocyte-specific gene expression and characteristic cellular micro-architecture. Overlay of matrix-preparations on de-differentiated hepatocytes restores differentiation. Integrin-linked kinase (ILK) is a cell-matrix-adhesion protein crucial in fundamental processes such as differentiation and survival. In this study, we investigated the role of ILK, and its binding partners PINCH, α-parvin, and Mig-2 in matrix-induced hepatocyte differentiation. We report here that ILK is present in the liver and localizes at cell-matrix adhesions of cultured hepatocytes. We also show that ILK, PINCH, α-parvin, and Mig-2 expression level is dramatically reduced in the re-differentiated hepatocytes. Interestingly, hepatocytes lacking ILK undergo matrix-induced differentiation but their differentiation is incomplete, as judged by monitoring cell morphology and production of albumin. Our results show that ILK and cell-matrix adhesion proteins play an important role in the process of matrix-induced hepatocyte differentiation.
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.titleIntegrin-linked kinase is involved in matrix-induced hepatocyte differentiation
elsevier.identifier.doi10.1016/j.bbrc.2006.12.091
elsevier.identifier.eid2-s2.0-33845989065
elsevier.identifier.piiS0006291X06027252
elsevier.identifier.scopusidSCOPUS_ID:33845989065
elsevier.volume353
elsevier.issue.identifier3
elsevier.coverdate2007-02-16
elsevier.coverdisplaydate16 February 2007
elsevier.openaccess0
elsevier.openaccessflagfalse
elsevier.aggregationtypeJournal


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